Presence of an endonuclease specific for ultraviolet light-irradiated deoxyribonucleic acid in a ultraviolet light-sensitive mouse cell mutant Q31.
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منابع مشابه
Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease.
The purification and properties of an ultraviolet (UV) repair endonuclease are described. The enzyme is induced by infection of cells of Escherichia coli with phage T4 and is missing from extracts of cells infected with the UV-sensitive and excision-defective mutant T4V(1). The enzyme attacks UV-irradiated deoxyribonucleic acid (DNA) containing either hydroxymethylcytosine or cytosine, but does...
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An endonuclease purified from Micrococcus luteus makes single-strand breaks in ultraviolet (UV)-irradiated, native deoxyribonucleic acid (DNA). The purified endonuclease is able to reactivate UV-inactivated transforming DNA of Haemophilus influenzae, especially when the DNA is assayed on a UV-sensitive mutant of H. influenzae. After extensive endonuclease action, there is a loss of transforming...
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An enconuclease activity that reacts with x-irradiated DNA is present in extracts of E. coli. By using centrifugal methods to monitor the conversion of the supercoiled, circular double-stranded DNA for phage phi-x-174 (replicative form) or PM2 to the relaxed circular form it was possible to quantitate the rate of radiation induced endonuclease-sensitive sites in the DNA. For every single-strand...
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The fate of ultraviolet-induced, thymine-containing dimers in the deoxyribonucleic acid (DNA) of Bacillus subtilis was investigated in both the wild type (UV(R)) and an ultraviolet light-sensitive (UV(S)) mutant. During incubation in the dark, dimers were excised from the DNA of the UV(R)B. subtilis, but remained in the DNA of the UV(S) mutant. About 40% of the excised dimers recovered in the w...
متن کاملUltraviolet light-induced mutation and deoxyribonucleic acid synthesis in Escherichia coli.
Exposure of a bacterial population to a mutagenic dose of ultraviolet light generally does not establish phenotypically mutant cells immediately. Rather, a period of subeultivation of the irradiated cells is required before newly induced mutants can be detected (Demeree, 1946). It has been postulated (see Witkin, 1956, for a recent discussion) that the delay between irradiation and phenotypic e...
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ورودعنوان ژورنال:
- Journal of radiation research
دوره 23 3 شماره
صفحات -
تاریخ انتشار 1982